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normal human breast tissue lysate  (Novus Biologicals)


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    Novus Biologicals normal human breast tissue lysate
    Normal Human Breast Tissue Lysate, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+human+breast+tissue+lysate/pm41023731-64-7-18?v=Novus+Biologicals
    Average 93 stars, based on 7 article reviews
    normal human breast tissue lysate - by Bioz Stars, 2026-07
    93/100 stars

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    Dishevelled-1 proteins are highly expressed in triple-negative breast cancer cell lines. Total mRNA was isolated from different breast cancer cell lines: human non-cancer mammary epithelial cell line (MCF10A and MCF12F), hormone receptor positive breast cancer cells (MCF-7, T-47D) and triple-negative breast cancer cell lines (MDA-MB-231, BT-549 and MDA-MB-468). (A) Real-time PCR (qRT-PCR) analysis of endogenous DVL-1 gene was performed using intron-spanning primers. All results are expressed as mean ± SEM and considered significant at *p < 0.05, **p < 0.01 and ***p < 0.001. (B) The protein expression patterns of endogenous DVL-1 were analysed by Western blotting in breast cells lines, as described above, along with breast normal whole tissue lysates (NT1, NT2, and NT3). The membranes were probed with two different DVL-1 specific antibodies (D3570; Sigma and sc-8025; Santa Cruz Biotechnology, Inc), and β-actin was included as a control (see Supplementary Fig. ).

    Journal: Scientific Reports

    Article Title: Acetylation of conserved DVL-1 lysines regulates its nuclear translocation and binding to gene promoters in triple-negative breast cancer

    doi: 10.1038/s41598-019-52723-3

    Figure Lengend Snippet: Dishevelled-1 proteins are highly expressed in triple-negative breast cancer cell lines. Total mRNA was isolated from different breast cancer cell lines: human non-cancer mammary epithelial cell line (MCF10A and MCF12F), hormone receptor positive breast cancer cells (MCF-7, T-47D) and triple-negative breast cancer cell lines (MDA-MB-231, BT-549 and MDA-MB-468). (A) Real-time PCR (qRT-PCR) analysis of endogenous DVL-1 gene was performed using intron-spanning primers. All results are expressed as mean ± SEM and considered significant at *p < 0.05, **p < 0.01 and ***p < 0.001. (B) The protein expression patterns of endogenous DVL-1 were analysed by Western blotting in breast cells lines, as described above, along with breast normal whole tissue lysates (NT1, NT2, and NT3). The membranes were probed with two different DVL-1 specific antibodies (D3570; Sigma and sc-8025; Santa Cruz Biotechnology, Inc), and β-actin was included as a control (see Supplementary Fig. ).

    Article Snippet: Human breast whole tissue lysates (normal) were purchased from GeneTex (GTX24006), Novus Biological (NB820-59203) and G-Biosciences (NLH-13).

    Techniques: Isolation, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Expressing, Western Blot, Control